Coding
TEVpC
Part:BBa_K2549015:Design
Designed by: Rongrong Du Group: iGEM18_Fudan (2018-10-06)
TEV protease C-terminal fragment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is C-terminal fragment of TEVp (Part:BBa_K2549013). In our project, TEVpC was fused to CfaC (Part:BBa_K2549010) intein, when co-expressed with CfaN (Part:BBa_K2549009) + TEVpN (Part:BBa_K2549014) in the same cell, will reconstitute site-specific protease activity.
Source
From IDT (gBlock), codon optimized for human